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lenticrisprv2 puro vector  (Addgene inc)


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    Structured Review

    Addgene inc lenticrisprv2 puro vector
    Lenticrisprv2 Puro Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 98/100, based on 517 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lenticrisprv2+plasmid/lentiCRISPRv2+puro+(Plasmid+%2398290)/pmc13007490-34-8-10
    Average 98 stars, based on 517 article reviews
    lenticrisprv2 puro vector - by Bioz Stars, 2026-09
    98/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Liver exerkine reverses aging- and Alzheimer's-related memory loss via vasculature.
    Article Snippet: AAV viral particles were purified and concentrated using Ultracentrifugation (Beckman Coulter). .. Genomic viral titers were measured using qPCR in combination with primer sets targeting the ITR sequence (FWD: GGAACCCCTAGTGATGGAGTT; REV CGGCC TCAGTGAGCGA).63 Abrogation of cerebrovascular TNAP in aged Cas9-inducible mice 5 different Alpl-targeting gRNAs and a non-targeting safe harbor control guide were first cloned into the lentiCRISPRv2 plasmid (lentiCRISPR v2 was a gift from Feng Zhang; Addgene plasmid #52961),64 packaged into lentiviral particles and used to transduce murine Neuro-2a cells. ..

    Sequencing:

    Article Title: Liver exerkine reverses aging- and Alzheimer's-related memory loss via vasculature.
    Article Snippet: AAV viral particles were purified and concentrated using Ultracentrifugation (Beckman Coulter). .. Genomic viral titers were measured using qPCR in combination with primer sets targeting the ITR sequence (FWD: GGAACCCCTAGTGATGGAGTT; REV CGGCC TCAGTGAGCGA).63 Abrogation of cerebrovascular TNAP in aged Cas9-inducible mice 5 different Alpl-targeting gRNAs and a non-targeting safe harbor control guide were first cloned into the lentiCRISPRv2 plasmid (lentiCRISPR v2 was a gift from Feng Zhang; Addgene plasmid #52961),64 packaged into lentiviral particles and used to transduce murine Neuro-2a cells. ..

    Control:

    Article Title: Liver exerkine reverses aging- and Alzheimer's-related memory loss via vasculature.
    Article Snippet: AAV viral particles were purified and concentrated using Ultracentrifugation (Beckman Coulter). .. Genomic viral titers were measured using qPCR in combination with primer sets targeting the ITR sequence (FWD: GGAACCCCTAGTGATGGAGTT; REV CGGCC TCAGTGAGCGA).63 Abrogation of cerebrovascular TNAP in aged Cas9-inducible mice 5 different Alpl-targeting gRNAs and a non-targeting safe harbor control guide were first cloned into the lentiCRISPRv2 plasmid (lentiCRISPR v2 was a gift from Feng Zhang; Addgene plasmid #52961),64 packaged into lentiviral particles and used to transduce murine Neuro-2a cells. ..

    Clone Assay:

    Article Title: Liver exerkine reverses aging- and Alzheimer's-related memory loss via vasculature.
    Article Snippet: AAV viral particles were purified and concentrated using Ultracentrifugation (Beckman Coulter). .. Genomic viral titers were measured using qPCR in combination with primer sets targeting the ITR sequence (FWD: GGAACCCCTAGTGATGGAGTT; REV CGGCC TCAGTGAGCGA).63 Abrogation of cerebrovascular TNAP in aged Cas9-inducible mice 5 different Alpl-targeting gRNAs and a non-targeting safe harbor control guide were first cloned into the lentiCRISPRv2 plasmid (lentiCRISPR v2 was a gift from Feng Zhang; Addgene plasmid #52961),64 packaged into lentiviral particles and used to transduce murine Neuro-2a cells. ..

    Article Title: Honokiol blocks tumor development and metastasis through mitochondrion-targeted effects.
    Article Snippet: .. Briefly, oligonucleotide pairs were annealed and cloned into lentiCRISPRv2 plasmid (Addgene, Watertown, MA, USA #52961) and cotransfected into HEK-293T cells, with the three packaging plasmids pMDLg/pRRE, pRSV-Rev and pMD2.G, for viral production. ..

    Article Title: Lysine lactylation regulates ATF4-mediated stress responses under glucose starvation in canine hemangiosarcoma.
    Article Snippet: .. Oligonucleotides for each selected sgRNA were synthesized, annealed, and cloned into the lentiCRISPRv2 plasmid (a gift from Feng Zhang, Addgene plasmid #52961; RRID: Addgene_52,961) (35) according to the provider’s protocol. ..

    Article Title: Integrative functional genomics analysis identifies pleiotropic genes for vascular diseases.
    Article Snippet: .. Corresponding oligonucleotides were synthetized by GenScript, and cloned into the lentiCRISPRv2 plasmid (Addgene, #52961) as described89. ..

    Article Title: Lysine lactylation regulates ATF4-mediated stress responses under glucose starvation in canine hemangiosarcoma
    Article Snippet: .. Oligonucleotides for each selected sgRNA were synthesized, annealed, and cloned into the lentiCRISPRv2 plasmid (a gift from Feng Zhang, Addgene plasmid #52961; RRID: Addgene_52,961) ( ) according to the provider’s protocol. ..

    Article Title: eIF4E and Ezrin cooperate in pseudopods to drive a localized migratory translation program in acute myeloid leukemia
    Article Snippet: All siRNA were from IDT Technologies. siRNA duplex_Luciferase sense (CACGUACGCGGAAUACUUCGAAATG) and antisense (CAUUUCGAAGUAUUCCGCGUACGUGUU) siRNA duplex _Ezrin sense (GGAAUCAACUAUUUCGAG) and antisense (UUUUUAUCUCGAAAUAGU); siRNA duplex eIF4E: sense (CUGCGUCAAGCAAUCGAGAUUUGGG) and antisense (CCCAAAUCUCGAUUGCUUGACGCAGUC). .. MM6 and THP-1 CRISPR clones with eIF4E disruption were generated using Lenticrisprv2 plasmid purchased from Addgene , . ..

    Plasmid Preparation:

    Article Title: Liver exerkine reverses aging- and Alzheimer's-related memory loss via vasculature.
    Article Snippet: AAV viral particles were purified and concentrated using Ultracentrifugation (Beckman Coulter). .. Genomic viral titers were measured using qPCR in combination with primer sets targeting the ITR sequence (FWD: GGAACCCCTAGTGATGGAGTT; REV CGGCC TCAGTGAGCGA).63 Abrogation of cerebrovascular TNAP in aged Cas9-inducible mice 5 different Alpl-targeting gRNAs and a non-targeting safe harbor control guide were first cloned into the lentiCRISPRv2 plasmid (lentiCRISPR v2 was a gift from Feng Zhang; Addgene plasmid #52961),64 packaged into lentiviral particles and used to transduce murine Neuro-2a cells. ..

    Article Title: Honokiol blocks tumor development and metastasis through mitochondrion-targeted effects.
    Article Snippet: .. Briefly, oligonucleotide pairs were annealed and cloned into lentiCRISPRv2 plasmid (Addgene, Watertown, MA, USA #52961) and cotransfected into HEK-293T cells, with the three packaging plasmids pMDLg/pRRE, pRSV-Rev and pMD2.G, for viral production. ..

    Article Title: GTPase Rab11b and effector Rab11-FIP2 promote NLRP3 stability during inflammasome priming
    Article Snippet: .. Generation of a stable THP-1 deficient in FIP2 expression To make the THP-1 FIP2 knock out cell lines a LentiCRISPRv2 plasmid was uses for transduction [PMID: 25075903] (gift from Feng Zhang lab - #52961 Addgene, Watertown, MA, USA). ..

    Article Title: ZNF121 recruits YTHDF2 to modulate mRNA stability
    Article Snippet: .. CRISPR-mediated gene knockouts were performed using the LentiCRISPRv2 plasmid, which was kindly provided by the Moffat Lab (Addgene plasmid #52961). .. Guide RNA (gRNA) sequences from the Toronto KnockOut version 3 (TKOv3) library for ZNF121 (AATGTGGAAGAGCCTTCGCT) and YTHDF2 (ATATAGGTCAGCCAACCCAG) were each cloned into the LentiCRISPRv2 vector and transfected into 293T cells to produce lentiviral particles Flp-InTMT-REx HEK293 cells were infected with lentivirus at an MOI of ∼0.3 in the presence of polybrene (8ug/mL; Sigma-Aldrich, Cat # H9268), selected with puromycin (2μg/mL; BioShop Canada Inc., Cat # PUR333.25) for 3 days and grown for 3 more days.

    Article Title: Lysine lactylation regulates ATF4-mediated stress responses under glucose starvation in canine hemangiosarcoma.
    Article Snippet: .. Oligonucleotides for each selected sgRNA were synthesized, annealed, and cloned into the lentiCRISPRv2 plasmid (a gift from Feng Zhang, Addgene plasmid #52961; RRID: Addgene_52,961) (35) according to the provider’s protocol. ..

    Article Title: Integrative functional genomics analysis identifies pleiotropic genes for vascular diseases.
    Article Snippet: .. Corresponding oligonucleotides were synthetized by GenScript, and cloned into the lentiCRISPRv2 plasmid (Addgene, #52961) as described89. ..

    Article Title: Lysine lactylation regulates ATF4-mediated stress responses under glucose starvation in canine hemangiosarcoma
    Article Snippet: .. Oligonucleotides for each selected sgRNA were synthesized, annealed, and cloned into the lentiCRISPRv2 plasmid (a gift from Feng Zhang, Addgene plasmid #52961; RRID: Addgene_52,961) ( ) according to the provider’s protocol. ..

    Article Title: eIF4E and Ezrin cooperate in pseudopods to drive a localized migratory translation program in acute myeloid leukemia
    Article Snippet: All siRNA were from IDT Technologies. siRNA duplex_Luciferase sense (CACGUACGCGGAAUACUUCGAAATG) and antisense (CAUUUCGAAGUAUUCCGCGUACGUGUU) siRNA duplex _Ezrin sense (GGAAUCAACUAUUUCGAG) and antisense (UUUUUAUCUCGAAAUAGU); siRNA duplex eIF4E: sense (CUGCGUCAAGCAAUCGAGAUUUGGG) and antisense (CCCAAAUCUCGAUUGCUUGACGCAGUC). .. MM6 and THP-1 CRISPR clones with eIF4E disruption were generated using Lenticrisprv2 plasmid purchased from Addgene , . ..

    Transduction:

    Article Title: Liver exerkine reverses aging- and Alzheimer's-related memory loss via vasculature.
    Article Snippet: AAV viral particles were purified and concentrated using Ultracentrifugation (Beckman Coulter). .. Genomic viral titers were measured using qPCR in combination with primer sets targeting the ITR sequence (FWD: GGAACCCCTAGTGATGGAGTT; REV CGGCC TCAGTGAGCGA).63 Abrogation of cerebrovascular TNAP in aged Cas9-inducible mice 5 different Alpl-targeting gRNAs and a non-targeting safe harbor control guide were first cloned into the lentiCRISPRv2 plasmid (lentiCRISPR v2 was a gift from Feng Zhang; Addgene plasmid #52961),64 packaged into lentiviral particles and used to transduce murine Neuro-2a cells. ..

    Article Title: GTPase Rab11b and effector Rab11-FIP2 promote NLRP3 stability during inflammasome priming
    Article Snippet: .. Generation of a stable THP-1 deficient in FIP2 expression To make the THP-1 FIP2 knock out cell lines a LentiCRISPRv2 plasmid was uses for transduction [PMID: 25075903] (gift from Feng Zhang lab - #52961 Addgene, Watertown, MA, USA). ..

    Expressing:

    Article Title: GTPase Rab11b and effector Rab11-FIP2 promote NLRP3 stability during inflammasome priming
    Article Snippet: .. Generation of a stable THP-1 deficient in FIP2 expression To make the THP-1 FIP2 knock out cell lines a LentiCRISPRv2 plasmid was uses for transduction [PMID: 25075903] (gift from Feng Zhang lab - #52961 Addgene, Watertown, MA, USA). ..

    Knock-Out:

    Article Title: GTPase Rab11b and effector Rab11-FIP2 promote NLRP3 stability during inflammasome priming
    Article Snippet: .. Generation of a stable THP-1 deficient in FIP2 expression To make the THP-1 FIP2 knock out cell lines a LentiCRISPRv2 plasmid was uses for transduction [PMID: 25075903] (gift from Feng Zhang lab - #52961 Addgene, Watertown, MA, USA). ..

    CRISPR:

    Article Title: ZNF121 recruits YTHDF2 to modulate mRNA stability
    Article Snippet: .. CRISPR-mediated gene knockouts were performed using the LentiCRISPRv2 plasmid, which was kindly provided by the Moffat Lab (Addgene plasmid #52961). .. Guide RNA (gRNA) sequences from the Toronto KnockOut version 3 (TKOv3) library for ZNF121 (AATGTGGAAGAGCCTTCGCT) and YTHDF2 (ATATAGGTCAGCCAACCCAG) were each cloned into the LentiCRISPRv2 vector and transfected into 293T cells to produce lentiviral particles Flp-InTMT-REx HEK293 cells were infected with lentivirus at an MOI of ∼0.3 in the presence of polybrene (8ug/mL; Sigma-Aldrich, Cat # H9268), selected with puromycin (2μg/mL; BioShop Canada Inc., Cat # PUR333.25) for 3 days and grown for 3 more days.

    Article Title: eIF4E and Ezrin cooperate in pseudopods to drive a localized migratory translation program in acute myeloid leukemia
    Article Snippet: All siRNA were from IDT Technologies. siRNA duplex_Luciferase sense (CACGUACGCGGAAUACUUCGAAATG) and antisense (CAUUUCGAAGUAUUCCGCGUACGUGUU) siRNA duplex _Ezrin sense (GGAAUCAACUAUUUCGAG) and antisense (UUUUUAUCUCGAAAUAGU); siRNA duplex eIF4E: sense (CUGCGUCAAGCAAUCGAGAUUUGGG) and antisense (CCCAAAUCUCGAUUGCUUGACGCAGUC). .. MM6 and THP-1 CRISPR clones with eIF4E disruption were generated using Lenticrisprv2 plasmid purchased from Addgene , . ..

    Synthesized:

    Article Title: Lysine lactylation regulates ATF4-mediated stress responses under glucose starvation in canine hemangiosarcoma.
    Article Snippet: .. Oligonucleotides for each selected sgRNA were synthesized, annealed, and cloned into the lentiCRISPRv2 plasmid (a gift from Feng Zhang, Addgene plasmid #52961; RRID: Addgene_52,961) (35) according to the provider’s protocol. ..

    Article Title: Lysine lactylation regulates ATF4-mediated stress responses under glucose starvation in canine hemangiosarcoma
    Article Snippet: .. Oligonucleotides for each selected sgRNA were synthesized, annealed, and cloned into the lentiCRISPRv2 plasmid (a gift from Feng Zhang, Addgene plasmid #52961; RRID: Addgene_52,961) ( ) according to the provider’s protocol. ..

    Disruption:

    Article Title: eIF4E and Ezrin cooperate in pseudopods to drive a localized migratory translation program in acute myeloid leukemia
    Article Snippet: All siRNA were from IDT Technologies. siRNA duplex_Luciferase sense (CACGUACGCGGAAUACUUCGAAATG) and antisense (CAUUUCGAAGUAUUCCGCGUACGUGUU) siRNA duplex _Ezrin sense (GGAAUCAACUAUUUCGAG) and antisense (UUUUUAUCUCGAAAUAGU); siRNA duplex eIF4E: sense (CUGCGUCAAGCAAUCGAGAUUUGGG) and antisense (CCCAAAUCUCGAUUGCUUGACGCAGUC). .. MM6 and THP-1 CRISPR clones with eIF4E disruption were generated using Lenticrisprv2 plasmid purchased from Addgene , . ..

    Generated:

    Article Title: eIF4E and Ezrin cooperate in pseudopods to drive a localized migratory translation program in acute myeloid leukemia
    Article Snippet: All siRNA were from IDT Technologies. siRNA duplex_Luciferase sense (CACGUACGCGGAAUACUUCGAAATG) and antisense (CAUUUCGAAGUAUUCCGCGUACGUGUU) siRNA duplex _Ezrin sense (GGAAUCAACUAUUUCGAG) and antisense (UUUUUAUCUCGAAAUAGU); siRNA duplex eIF4E: sense (CUGCGUCAAGCAAUCGAGAUUUGGG) and antisense (CCCAAAUCUCGAUUGCUUGACGCAGUC). .. MM6 and THP-1 CRISPR clones with eIF4E disruption were generated using Lenticrisprv2 plasmid purchased from Addgene , . ..



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